Convenient Synthesis and Molecular Docking of Novel Pyrido [2,3- d ]pyrimidines as Potent Antimicrobial Candidates.

New 3-(3,4-dimethoxyphenyl)-1-(thiophene-2-yl)prop-2-en-1-one has been designed as a starting compound to synthesis novel series of substituted pyrido[2,3-d]pyrimidine system incorporated different Schiff's bases and enamine derivatives potent antimicrobial compounds. Novel synthesized compounds were evaluated for their in-vitro potency against gram-positive gram-negative bacteria, where they reveal high effectuality at low concentration compared Trimethoprim. Docking studies structure-activity relationship declared that new pyrido [2,3-d] pyrimidines completely occupied the active pocket Biotin Carboxylase both bacterial types fungal strains acting selective Fatty Acid Synthesase type II inhibitors.